Biofluorimetry Lab 

Dipartimento di Fisiopatologia Medica

University of Rome 'La Sapienza'

page created and maintained by Leopoldo Silvestroni & Simonetta Palleschi

last update May 15, 2006

--- active, but still heavily under construction ---

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MOVIES

actin filament generation in protruding and contractile assemblies

actin filament organization in the bottom half of the cell (movie)

actin filament organization in the top half of the cell (movie)

adhesion to substrate : the feet of a moving cell

aggregation behavior of hyperscattering cells

Bohr atom

calcium response / calcium recovery

Cell Motility - a video tour:

introduction : cytoskeleton

front and rear wheel drive

the actin cytoskeleton

the lamellipodium is an autonomous organelle of protrusion

actin polymerisation drives protrusion

the structural basis of protrusion

the generation of actin filaments in protruding and contractile assemblies

substrate adhesions : the feet of a moving cell

regulation of adhesion formation: Rho GTPases

focal adhesion formation is dependent on mechanical stress in the actin cytoskeleton

cell polarisation and microtubules

mt target focal adhesions

targeting occurs in the nanometer range

mt targeting correlates with substrate adhesion turnover or destabilisation

mt potentiate focal adhesion disassembly by inhibiting contractility

imposition of a gradient of contractility restores polarity in cells lacking mt

a working model of how microtubules polarise migrating cells

interim open questions

mt tip complexes offer an obvious signaling platform

the microtubule motor "kinesin-1" is involved in signal transmission

mt polymerisation is stimulated by mechanical stress in the actin cytoskeleton

cross-talk: tipping the balance of rho gtpases

cell polarisation and microtubules

chemotaxis of neutrophil chasing a bacterium (AVI – MOV)

cytoskeletal proteins in S-2 cells

Cytoskeletal Protein (in green) During Phagocytosis

Cytotoxic T-cell Killing Its Target (with sound)

dynamics of GFP-Zyxin and traction forces during cell migration (movie)

dynamics of the "molecular feet" of a migrating connective tissue cell (5.6 MB)

ER dynamics during inhibition of MT dynamics

ER extensions in a fish epidermal cell
ER-tubule extension by sliding along a microtubule

fibroblasts migrate away from compressing forces (movie)

fibroblasts migrate away from soft substrates (movie)

fibroblasts migrate toward stiff substrates (movie)

fibroblasts migrate toward stretching forces (movie)

flight through the animal cell

focal adhesion formation is dependent on mechanical stress in the actin

focal adhesion, traction forcess (Yu-Li Wang link)

formation and alignment of Z lines in developing myotubes (1) (2) (3)

future muscle cells migrating from their birth places toward a limb (875 KB)

Future nerve cells migrating toward their residence in the brain (3 MB)

global warming & the greenhouse effect
green fluorescent protein-tagged receptors in living cells undergoing chemotaxis (AVI – MOV)

human genome project

inhibition of motility by GTPγS

interim open questions

journey into DNA

kinesin, single molecule

kinesin-1microtubule motor is involved in signal transmission

Macrophages Taking a Walk

mammalian cells sense pulsating, near-infrared light sources

melanosomes moving on a microtubule carpet in vitro

microarray video

microtubule and ER dynamics in the lamella of a migrating cell

microtubule bending and breakage in the lamella

microtubule dynamics and retrograde flow seen by multi-mode microscopy

microtubule dynamics during protrusion of the leading edge

microtubule gliding

microtubule gliding in vitro

microtubule organization in dividing cells (movie)

microtubule tip complexes offer an obvious signaling platform

microtubules polarise migrating cells (how-)

microtubules potentiate focal adhesion disassembly by inhibiting contractility

Migration of fish epidermal cell
Mitochondria and lysosomes moving in fish epidermal cell

mitosis & meiosis

motility of chemotactically stimulated talin-null cells viewed by RICM (1.8 MB)

motility of chemotactically stimulated wild-type cells viewed by RICM (1.4 MB)

Movement of Alpha-Actinin Away from the Distal End of Stress Fibers (movie) 

movement of caveolae in CHO cells stably transfected with caveolin-1-GTP

movement of surface receptors during normal division (movie)

muscle (human) cells migration in culture and cell-cell contacts/"talk"

Nerve extending process. (159 KB)

neutrophil chasing a bacterium compared to a cell sheet during epithelial wound closure

phase images of a normal dividing cell (movie)

phase wildtype mitosis

Plant Root Hair Cell Growing
poisons:

cytochalasin effect on the movement of surface receptors (movie)

nocodazole effect on division (movie)

nocodazole effects on the movement of surface receptors (movie)

taxol effects on the movement of surface receptors (movie)

plant mitosis (7.6 MB)
animal mitosis (2.7 MB)
yeast mitosis

Retrograde movement of actin in fish epidermal cell

"Ruffling" of cell fragment from fish epidermal cell

ruffling microplasts prepared from human skin fibroblasts (1)(2)
sea urchin Cleavage Stage Embryos

stress fiber collapse upon activation with phorbol esters (HR movie)

stress fiber collapse upon ATP depletion (HR movie)

stress fiber recovery upon drug removal (HR movie)

targeting occurs in the nanometer range

telomeres and telomerase, visit the image gallery

visualization of gene expression in living cells

visualizing the central dogma in living cells: DNA → RNA → Protein

White Blood Cells Eating Yeast (with - brass - sound)
wildtype cytokinesis

wildtype mitosis (1) (2) (3)

ANIMATIONS

 action of epinephrine on a liver cell  (narrated)

action of thrombin on fibrinogen

action potential propagation in an unmyelinated axon  (narrated)

ATP synthesis

autonomous movements of cytoplasmic fragments

 bidirectional replication of dna  (narrated)

Boltzman distribution

can cells see each other?

cancer metastasis

carbon cycle

cardiac output

 cDNA (narrated)

cell collision behavior

cell cycle, apoptosis and hpv

cell division blocked by tumor suppressor genes (narrated)

cell migration in group

cell movement does not obey a 'law of refraction'

cell proliferation signaling pathway  (narrated)

cells detect microscopic infrared light sources

cells detect subtle guidance

cells probe their surroundings

cellular organelle evolution

centrioles: the 'eye' of the cell?

cervical neoplasia

changes in the partial pressures of oxygen and carbon dioxide  (narrated)

  chemical synapse  (narrated)

 combination of switches the lac operon  (narrated)

comparison of hemodynamics in shock (self test)

components of the cell

 conjugation with f plasmid  (narrated)

 constructing vaccines (narrated)

 control of gene expression in eukaryotes  (narrated)

comparison of meiosis and mitosis  (narrated)

control of the cell cycle  (narrated)

cotransport (narrated)

crossing-over

cyclic and noncyclic photophosphorylation (narrated)

cytotoxic Tcell activity against target cells  (narrated)

DNA replication

DNA replication:

basic replication details

leading strand replication

lagging strand replication

lagging strand details

half replication bubble

full replication bubble

 DNA replication fork (narrated)

 early genetic engineering experiment (narrated)

effect of sound waves on cochlear structures  (narrated)

electrochemical cell (1) (2) (3) (4)

electron transport chain

electron transport system and ATP synthesis (narrated)

electrophoresis

endocytosis and exocytosis (narrated)

eye anatomy

feedback inhibition of biochemical pathways (narrated)

fertilization of mammialian egg

function of the neuromuscular junction  (narrated)

gamete diversity and independent gene assortment

geriatric case study (self test)

glycolysis

hand kinesiology

heart transplant

heat changes protein structure

herpes simplex virus infection

hershey and chase experiment  (narrated)

histone acetylation and deacetylation

  HIV replication  (narrated)

HLA haplotype inheritance

how intracellular receptors regulate gene transcription  (narrated)

 how nucleotides are added in dna replication (narrated)

  how prions arise  (narrated)

IgE mediated hypersensitivity  (narrated)

in vitro fertilization

infrared detection is not phototaxis

integration and excision of a plasmid  (narrated)

interaction of antigen presenting cells and thelper cells  (narrated)

IP3 and Ca2+ ions as second messengers (with sound)

IV pump simulations

Jablonski diagram

kidney at work

kinesin-1 walking along a microtubule

kinesin-1 walking models

leukocytes adhering to endothelium

light penetration of the skin

lymphocyte toxicity test

lysosomes (narrated)

  malaria life cycle of plasmodium  (narrated)

MAP kinase signal transduction pathway (with sound)

mechanism of steroid hormone action  (narrated)

mechanism of thyroxine action  (narrated)

mechanism of transposition  (narrated)

meiosis

  membrane bound receptors G proteins and Ca2 channels  (narrated)

 Meselson and Stahl experiment  (narrated)

Mendel peas

MHC class I

MHC class II

  microarray (narrated)

microtubules: the 'nerves' of the cell?

mitosis

mitosis

mitosis (working)

 mitosis and cytokinesis  (narrated)

monoclonal antibody production  (narrated)

mRNA splicing

natural selection

near-infrared vision in the social behaviorof cells

nerve synapses and signalling

neuromuscular blockade

nitrogen cycle

normal alveolus

nucleic acids

osteoclast in 3D

ovarian & uterine cycles

oxidative phosphorylation

passive diffusion and active uptake

paternal testing

PCR

PCR

PCR

 PCR (narrated)
 photosynthetic electron transport and ATP synthesis  (narrated)

personality disorder game

phagocytic oxygen dependent killing effects

photon absorption and transfer of energy

photosynthesis

 processing of gene information prokaryotes versus eukaryotes (narrated)

 protein synthesis (narrated)

protein folding

protein sorting

protein sorting (single pass)

protein synthesis

 protein transport

proton pump (narrated)

 random orientation of chromosomes during meiosis  (narrated)

 RNA processed by  spliceosomes (narrated)

 restriction endonucleases (narrated)

 restriction fragment length polymorphisms (narrated)

reactions of metals and metal ions

regulation of physiologic fibrinolysis

renal tubular cell

 rtPCR  (with sound)

  sarcomere shortening  (narrated)

 Southern blot (narrated)

 second messengers the cAMP and Ca pathways  (narrated)

sea urchin gastrulation

sex determination & linkage

signal amplification  (narrated)

  sodium potassium exchange  (narrated)

sodium potassium exchange pump (narrated)

 slippedstrand mispairing  (narrated)

 spermatogenesis  (narrated)

 stages of meiosis  (narrated)

stem cells

steps in cloning a gene (narrated)

 the Ti plasmid  (narrated)

 the tryptophan repressor  (narrated)

 transcription complex and enhancers  (narrated)

 the consequence of inversion  (narrated)

 thymine dimers  (narrated)

 the consequence of inversion  (narrated)

symmetry of sister cells

T-dependent humoral response

tissue oxygenation tutorial

  three phases of gastric secretion  (narrated)

transcription

 transcription by RNA polymerase

 treatment of HIV  (narrated)

  unique features of meiosis  (narrated)

translation

  voltage gated channels and the action potential  (narrated)

yeast cell cycle

WNT pathway

IMAGES

actin and tubulin probes

bioimage database

bone marrow and hemopoiesis

cartilage and bone and bone histogenesis

catalysis

cell mobility and cytoskeleton

circulatory system

connective tissue

crystallization

cytoskeleton

Dennis Kunkel microscopy (beginners)

digestive system: alimentary canal

digestive system: oral cavity and teeth

DNA

endocrine system

epithelial tissue, surface specializations, and glands

eye

female reproductive system (including organs of pregnancy)

histology learning system

Histology slide list:

immunofluorescence image gallery

immunohistochemistry image gallery

in situ hybridization image gallery

integument

Invitrogen - Molecular Probes - gallery (searchable)

liver, gall bladder, and pancreas

male reproductive system

lymphocyte traffic and homing

lymphoid tissues and organs

macromolecules

muscle tissue

nanotechnology

Nanoworld

nervous tissue and neuromuscular junction

PDB Lite: find & download macromolecule files

peripheral blood cells

polymers

respiratory system

 small molecules

solid state

 tissues, layers, and organs

 ultrastructure of the cell

urinary system

web atlas of cellular structures using light and confocal microscopy

main sourcesfor this page

Accelrys - Biomedical Engineering department at the University of California Irvine - Cell Web Front Page - CELL INTELLIGENCE - Dr Mark Hill,The University of New South Wales, Sydney - Mrs Balling TeacherWeb - Spectorlab - The Vale Lab Home Page - Thomas J. Greenbowe, Department of Chemistry, Iowa State University -