welcome to the home page of the
Biofluorimetry Lab
chief: Leopoldo Silvestroni,MD


 Dipartimento di Fisiopatologia Medica
La Sapienza Universitą di Roma
Rome, ITALY

 

last update July 16, 2011

 

 main site pages

 

we on YOUTUBE
ATOMIC FORCE MICROSCOPY SPERM IN CERVICAL MUCUS
ANIMATED ACROSOME REACTION (SCANNING ELECTRON MICROSCOPY) CONTRACTION OF TESTICULAR TUBULES
LIVE LEUKOCYTES SPERM IN SEMEN
"BABY" IN SPERM HUNGRY LEUKOCYTES
DIAGNOSTICS OF HUMAN SPERM ACROSOMES  

 


Main Site Pages

Analysis of Data and Statistics

At the lab bench & Safety

Bioethics

Cell preparation & Culturing

Chemistry

Endocrine Disrupting Chemicals

Fluorescence & Spectroscopy

Microscopy

Nucleic acids and Genetics

Physics & Biophysics

Toxicology & Environment

Please note:
The above pages also link You to titles and abstracts of the last six-months articles in leading Journals: enjoy it!


Italian area

 

La sicurezza dei dati sanitari 

 

past event

Ministero della salute

Endometriosi. Percorsi e qualitą di vita

June 15, 2005

Istituto Superiore di Sanitą - Aula Magna

Viale Regina Elena 299, Roma

Program (1) (2)

 

past event

Ministero della salute

Patologie andrologiche dell'etą giovanile:

il ruolo della prevenzione

December 15, 2004

Camera dei Deputati - Sala delle Conferenze di Palazzo Marini

Via del Pozzetto 158, Roma

programma e modulo di iscrizione

Gli Atti del convegno

sono pubblicati nel portale del

Ministero della Salute

consulta e/o scarica gli Atti

 

past event

Ministero della salute

Vivere in dialisi: dalla qualitą dei servizi alla qualitą della vita

April 26, 2004

Camera dei Deputati - Sala del Cenacolo

Palazzo Valdina

Piazza in Campo Marzio, 42 - Roma

 

Gli Atti del convegno

sono pubblicati nel portale del

Ministero della Salute

consulta e/o scarica gli Atti

 

past event

Fertilitą e Ambiente - Scienza e Societą

Roma, 22 Novembre 2003


Our Lab

Instruments and technologies

Laboratory equipment, product & reagent suppliers page

Photobook page

Publications page

Repository of (bio)chemical reagents page ()

Scientific interests and research projects

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BioMedNet

 

PubMed  NLM Databases  TOXNET

 

sperm bibliography update

 

 

journals impact factors

full text journals

(local users only)


 INSTRUMENTS AND TECHNOLOGIES

Optical microscope Olympus IX70 (bright field, phase contrast, epifluorescence, photography, CCD BW/color cameras, monitor, Pinnacle System, VCR).

            Philips E500 Scanning Electron Microscope (Dipartimento di Ingegneria Chimica, Universitą di Roma 'La Sapienza')

 

(visit our MICROSCOPY & PHOTOBOOK pages !)

Atomic Force Microscope NANONICS (AFM and SNOM)

Hamamatsu PMA-11 Multiwavelength Spectrometer, 300-800 nm low-level energies collection, displaying and filing. It is adapted to the Olympus IX70 microscope

Perkin Elmer UV/VIS spectrophotometer

Kontron SFM25 spectrofluorometer, computer driven, flow cuvettes for fluorescence readings both in cell suspensions and monolayers, polarizers, cell stirrer, temperature control

(visit our FLUORESCENCE / SPECTROSCOPY page !)

Electrophoresis: standard/mini mono/bidimensional agar/SDS-PAGE / polyacrylamide slabs and rod gels, isoelectrofocusing, immunoelectrophoresis, electroblotting, autoradiography

Centrifugation / Ultracentrifugation

Hardware: Pentium4 computers, BW/color monitors, 2940 U/W SCSI adapters, CD-DVD players, CD-DVD recorder, laser and digital color printer, flat scanners, audio station, image acquisition (from microscope, monitor and VCR), Pinnacle board, Videoserver

Software: general purpose, scientific packages for data acquisition and image analysis


 LIST OF CHEMICAL AND BIOLOGICAL REAGENTS AVAILABLE IN OUR LAB  (in italian)

Italian area

 

La sicurezza dei dati sanitari 

 

past event

Ministero della salute

Endometriosi. Percorsi e qualitą di vita

June 15, 2005

Istituto Superiore di Sanitą - Aula Magna

Viale Regina Elena 299, Roma

Program (1) (2)

 

past event

Ministero della salute

Patologie andrologiche dell'etą giovanile:

il ruolo della prevenzione

December 15, 2004

Camera dei Deputati - Sala delle Conferenze di Palazzo Marini

Via del Pozzetto 158, Roma

programma e modulo di iscrizione

Gli Atti del convegno

sono pubblicati nel portale del

Ministero della Salute

consulta e/o scarica gli Atti

 

past event

Ministero della salute

Vivere in dialisi: dalla qualitą dei servizi alla qualitą della vita

April 26, 2004

Camera dei Deputati - Sala del Cenacolo

Palazzo Valdina

Piazza in Campo Marzio, 42 - Roma

 

Gli Atti del convegno

sono pubblicati nel portale del

Ministero della Salute

consulta e/o scarica gli Atti

 

past event

Fertilitą e Ambiente - Scienza e Societą

Roma, 22 Novembre 2003


 

Our lab work concentrates also on the toxic potential of

endocrine disrupting chemicals - EDCs


SCIENTIFIC INTERESTS AND RESEARCH PROJECTS
Human sperm physiology
Male gamete functions represent a cultural challenge for either basic and clinical investigators. As a matter of fact, though an ever growing body of knowledge is accumulating on this topic, how and where spermatozoa acquire fertilizing ability and motility, what molecular mechanisms underlie the development of capacitation into the female genital tract, as well as the precise correlations between sperm functional status and the capacity of undergoing an acrosome reaction at the site of fertilization are some still unaswered fundamental questions.


Health effects of environmental pollutants


in collaboration with and funded in part by Istituto Superiore di Sanitą Research projects:

1) Prevention of Risk Factors of Child and Maternal Health
2) Human Exposure to Xenobiotics with Potential Endocrine Activities: Evaluation of the Risks for Reproduction and Development

Two lines of research are in progress in our lab aiming to identify and characterize the effects of some man-made organic chemicals (compounds endowed with hormonal activity/endocrine effects - the so-called xenoestrogens or hormone disruptors) on human spermatozoa and rat testicular cells.

The compounds under study are:
phthalates
alkylphenols
bisphenol A
polychlorinated biphenyls (PCB / Aroclors)
organochlorine insecticides
dioxins
lindane

 


 

 

accesses since January 15,1998

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page created by Leopoldo Silvestroni and Simonetta Palleschi


 

 

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