Fluorescence - Spectroscopy

last update March 3, 2007

 

See also our At the lab bench, Chemistry & Microscopy  pages

accesses from September 15, 1998:

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the fluorometer	the multiwavelength spectrometer

excitation and emission wavelenghts
speed of wavelenght changes
phototube sensitivity
time-based readings collection
mesaurements

A series of programs (BASIC & LabView) have been developed to measure:

intracellular calcium concentration (Quin2, Fura2,Indo1)

intracellular pH (BCECF)

membrane potential (bisoxonol)

cell viability (propidium iodide)

protease activity (BAAMC)

endocytosis and exocytosis (lucifer yellow, TMA-DPH)

membrane fluidity (Laurdan,Prodan)

energy transfer

Other programs are dedicated to fluorescence polarization:

membrane fluidity (DPH,TMA-DPH)

binding of fluoresceinated (FITC-labelled) ligands

Applied Electromagnetics and Optics Laboratory
Atomic Absorption Spectroscopy
Atomic Emission Spectroscopy
Atomic fluorescence spectroscopy

Atomic spectra database (levels query form)
Atomic spectroscopy
Atomic Spectroscopy Theory (in: Analytical spectroscopy)
Autofluorescence in cells and tissues
Beer-Lambert Law
Ca measuremement (remote with a contact probe; hearth)
Calibration (in situ) of intracellular [Ca++]i, [Na+]i and pHi
CCD intensified cameras (from OBB) - CCD Products (much more in our Microscopy  page)

Cell Organelles (see Images in our Microscopy  page):

Nucleus

Endoplasmic reticulum & Mitochondria

Cytoskeleton (microtubules & actin)

Lysosomes

Images of double labeling

Images of triple labeling

Center for fluorescence fpectroscopy (Dr. J.R. Lakowicz)
Choppers

Circular Dichroism Specroscopy
CyDye fluorescent labels
Electrodynamic Theory of Fluorescence Polarization
Electromagnetic Radiation
Electromagnetic Spectrum
Environmental sensitivity of fluorescence
Fiberoptic/liquid light guides
Fiberoptic remote Ca measurement (hearth)
Filter wheels
Flow Cytometry Sites

Fluorescence Correlation Spectroscopy (1) (2) (3) (4)

Fluorescence glossary

Fluorescence Imaging Techniques (1) (automation in, 2) (3)
Fluorescence in biology
Fluorescence In-Situ Hybridization
Fluorescence lifetime applications
Fluorescence measurement in muscle strips
Fluorescence polarization
Fluorescence Polarization Discussion Group
Flourescence Recovery After Photobleaching (FRAP) (1) (2) (3) (4)

Fluorescence Resonance Energy Transfer - FRET - (1) (2) (PDF, 3) (Imaging) (PDF) (dipole-dipole excitation transfer)
Fluorescence spectra (emission-shifted dyes)
Fluorescence spectra (excitation-shifted dyes)
Fluorescence spectra (dual-fluorescent dyes)
Fluorescence spectra (non ratiometric dyes)

Fluorescence spectroscopy
Fluorescence spectroscopy accessories
Fluorescence spectroscopy: advantages from scanning monochromator
Fluorescence spectroscopy: sensitivity measurement, noise, ...
Fluorescent Dyes for Two-Photon Microscopy

Fluorescent probes: physics
Fluorochrome List and Spectral Properties

Forster (see Fluorescence Resonance Energy Transfer)
Green Fluorescent Proteins (molecular structure)
History of fluorescence
ICQEM-CNR Spectroscopy Lab
Imaging (lifetime)

Infrared Spectroscopy - basic

Interaction of Light with Matter
Intracellular Calcium measurement
Introduction to Spectroscopy and Polarization

IUPAC Gold Book (>6,000 items)
Journal of Fluorescence
Laboratory for Fluorescence Dynamics (Prof. W.W. Mantulin)
Laser dyes (1) (2) (3)
Laser tutorial
Lifetime fluorescence spectroscopy
Light Absorption

Light detection
Light guide specification sheet
Light measurement handbook (PDF, 1) (PDF,  2)
Light-sample handling
Light sources
Molecular Wavefunction
Monochromator gratings
Monochromators

Multiphoton dye spectra
Multi-wavelength illuminator
Optical Fibers and Light Guides (Reference Guide)
Optical Imaging / Photon Migration
Optical Societies
pH measurement with BCECF: improving sensitivity
Photon counting
Quantitative Fluorimetry

Quantum Mechanics
Quantum Mechanics - Wavefunctions
Raman microscopy
Raman spectroscopy
Scattering
Society for Applied Spectroscopy
Software for Mass Spectrometry
Software for Time / Frequency Domain Fluorescence Lifetime Analysis
Spectra databases (1) (atomic, 2)
Spectral emission of lamps (Xe, Xe/Hg, Tungsten halogen)
Spectroscopic Effects

Specrtosopy Net
Spectroscopy technical notes
Spectrophotometry (1) (2)
Stepper Motor Driver (from PTI)
Two-photon microscopy (1) (2) (3) (PDF, 4)

PhotochemCAD (Computer Aided Photochemistry)

Fluorescent Dye Spectra

SPECTRA OF SINGLE FLUORESCENT PROBES

Acid Blue 40
Alizarin

Allophycocyanin/pH 7.5
7-Aminoactinomycin D/DNA
BCECF/pH 9.0 and 5.5
BOBO-1/DNA
BODIPY 630/650-X/MeOH

Brilliant Green
Bromocresol Green
Bromothymol Blue
Chicago Sky Blue
Chrysophenine
Congo Red
Cresol Red
Crystal Violet
Direct Red 81
Direct Yellow 8
Direct Yellow 50
Disperse Red 19
Disperse Yellow 3
Eosin Y
Erythrosin B
Ethyl Violet
Evan's Blue
Fast Black
Indoine Blue
Janus Green B
Martius Yellow
Methyl Orange
Methyl Red
Methylene Blue
Methylene Green
Methylene Violet
Napthol Blue Black
New Methylene Blue N
Phenolplthalein
Phenol Red
Quinaldine Red
Thymol Blue
Thymolphthalein

...TO BE COMPLETED...
 
 

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